We aim to democratize microscopy, reduce the reproduction crisis and enhance trust into science by making it available to everyone via … MERIT Errantry as a chance for the long-term overseas dispatch I joined a research group of Dr. Mosig at Friedrich Schiller University Jena in Germany for 2 months from September 2015. These authors contributed equally to this work.Correspondence: Bachstrasse 18, 07743 Jena, Germany. alexander.mosig@med.uni-jena.de. The purpose of the present study was to investigate the effects of acute exercise on gene expression profiles (GEPs) of WBCs and to identify suitable genes that may serve as surrogate markers for monitoring exercise and... Ziel dieser Arbeit war es, anhand der Untersuchung von Genexpressionsprofilen (GEP) von Monozyten und T-Zellen weitergehende Erkenntnisse über die molekularen und zellulären Vorgänge in diesen Zellen bei der Entwicklung der Atherosklerose von Patienten mit Familiärer Hypercholesterinämie zu erhalten. Image: Jan-Peter Kasper (University of Jena) Lichtwerkstatt - Open Photonics Makerspace. FASEB J.. Mosig. CD91/Lrp1 is not involved in nLDL uptake. 3 Center for Electron Microscopy, Jena University Hospital, Friedrich Schiller University Jena, Ziegelmuehlenweg 1, 07743 Jena, Germany; frank.steiniger@med.uni-jena.de 4 Center for Sepsis Control and Care, Institute of Biochemistry II, Jena University Hospital, (2014). Raman spectroscopic imaging was used to visualize the cell nuclei by means of false color imaging using a marker band, and a cell segmentation w... Complex cell culture models such as microphysiological models (MPS) mimicking human liver functionality in vitro are in the spotlight as alternative to conventional cell culture and animal models. The liver is a central organ in the metabolization of nutrition, endogenous and exogenous substances, and xenobiotic drugs. Identification of high fat diet induced inflammatory gene networks associated with suppression of anti-inflammatory phospholipids in human adipose tissue in the NUGAT (Nutrigenomics Analysis in Twins) study, Microfluidically supported biochip design for culture of endothelial cell layers with improved perfusion conditions, A microfluidically perfused three dimensional human liver model, Optimization of the transfection of human THP-1 macrophages by application of Nunc UpCell technology, Cell type-specific delivery of short interfering RNAs by dye-functionalised theranostic nanoparticles, High fat diet induced adipose tissue inflammation by suppression of anti-inflammatory phospholipids, Identification of gene-networks associated with specific lipid metabolites by Weighted Gene Co-Expression Network Analysis (WGCNA), Microfluidic devices for cell culture and handling in organ-on-a-chip applications, Long-chain metabolites of α-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro, SORBS2 and TLR3 induce premature senescence in primary human fibroblasts and keratinocytes, Analysis of glucose and fetal calf serum in aqueous solution for Multi Organ Tissue Flow (MOTiF) bioreactors using NIR Spectroscopy, Towards an understanding of the interaction between liver cells and antibiotics via Raman microspectroscopy, Antioxidative and Non-Antioxidative Effects of Tocopherol Metabolites on Atherogenesis, METHOD AND DEVICE FOR DETECTING THE MOTION AND ADSORPTION OF CELLS AND PARTICLES ON CELL, TISSUE, AND IMPLANT LAYERS IN THE SIMULATION OF FLOW CONDITIONS, Verfahren und Vorrichtung zur Erfassung der Bewegung und Anlagerung von Zellen und Partikeln an Zell-, Gewebe- und Implantatschichten bei der Simulation von Flussbedingungen, Gene Expression And Autologous Blood Transfusion - A New Advance Towards Blood Doping Detection? The toolboxes are named according to the SPM version. Cell type-specific delivery of short interfering RNAs by dye-functionalised theranostic nanoparticles. Marko Gröger. Aim of this study was to functio... Background human liver sinusoid. University of Jena Teaching Research Team Faculty Back Team Microeconomics Team Team Information about our team. Increased CD36 and CD91/Lrp1 levels on the surface of monocytes from FH patients and their role in LDL uptake. Francisella tularensis, a gram-negative bacterium replicates intracellularly within macrophages and efficiently evades the innate immune response. Nevertheless, Jena's outstanding competence in high-frequency engineering paved the way to laser technology and, thus, the rise of optics and photonics to one of the most important fields of research at the Friedrich Schiller University Jena. Homepage Foto Image: shutterstock Institute; Departments; Research; Studies; Share this page. In this work, Raman spectroscopic cell imaging approaches and a discrimination between HepG2, nondifferentiated hepatic stem cell line HepaRG, and differentiated hepatocyte‐like HepaRG cells are presented. Mit dem Benutzen der Webseite erklären Sie sich der Nutzung von Cookies einverstanden. The development of therapeutic substances to treat diseases of the central nervous system is hampered by the tightness and selectivity of the blood-brain barrier. Image: Jan-Peter Kasper (University of Jena) Funding your research at ACP. University of Jena de; de; Institute Departments Research Studies Homepage Institute Departments Research Studies Welcome to the Institute of Sociology. 81 35392 Gießen Office: Carl-Zeiss-Str. Pneumonia is one of the most common infectious diseases worldwide. Long-term overseas dispatch program in Friedrich Schiller University Jena D2 Kaori Machitani Takeda 1. The new fluorescent mega Stokes dye (peak excitati... Polymer-based nanoparticles are promising drug delivery systems allowing the development of new drug and treatment strategies with reduced side effects. The data provided show that NPC1 as well as ABCA1 expression is deceased in FH monocytes. Gianni Panagiotou. Mit dem Benutzen der Webseite erklären Sie sich der Nutzung von Cookies einverstanden. sandy.mosig@mti.uni-jena.de The study was undertaken to investigate whether the two major monocyte subsets defined by the surface markers CD14(+)CD16(+) and CD14(++)CD16(-) show differences in their responses to hypercholesterolemia. A: legend of array index. Institute of Forensic Medicine, Jena University Hospital, 07747 Jena, Germany. E-mail address: sandy.mosig@mti.uni‐jena.de. Alexander MOSIG, Research Group Leader INSPIRE of Universitätsklinikum Jena, Jena | Read 88 publications | Contact Alexander MOSIG In the presence of ECs a lipopolysaccharide (LPS)-stimulus led to a significant increase... Liver dysfunction is an early event in sepsis-related multi-organ failure. The system is based on concepts of modular development, rapid-prototyping and all-time accessibility using widely available off-the-shelf optic and electronic components. Within the liver, non-parenchymal cells (NPCs) are critically involved in the regulation of hepatocyte polarization and maintenance of metabolic function. Kai Papenfort. For postdocs and staff, there … The use of these cell-based systems will allow to better understand in-vivo processes and to test for the direct influence of different substances on cell viability or metabolic activity e.g. Using mass spectrometry, we identified a C-terminal fragment of alpha-1-antitrypsin, designated CAAP48, as a new sepsis biomarker that actively participates in the pathophysiology... We present a novel open-source modular electro-optical toolbox to be used for many different optical setups. E‐mail: sandy.mosig@mti.uni‐jena.de Search for more papers by this author Histogram bars indicate the mean ± SD. A prerequisite for clonal expansion of cancer cells is unlimited proliferative capacity. 2008 - 2012 University Professor (W2) for Nutritional Biochemistry, Institute of Nutrition, FSU Jena 2006 Habilitation and venia legendi in biochemistry and cell biology, University of Münster 2003 - 2008 Head of Research Group, Leibniz Institute of Arteriosclerosis Research Münster The City of Jena. EOC-type-II tumors were analyzed for gene methy... HepaRG cells are widely used as an in vitro model to assess drug-induced hepatotoxicity. Weitere Pflichtangaben finden Sie in unserer Datenschutzerklärung. (2) ... 07747 Jena, Germany. S., et al. Harvesting cultivated macrophages for tissue engineering purposes by enzymatic digestion of cell adhesion molecules can potentially result in unintended activation, altered function, or behavior of these cells. Learn more. Conventional stationary cell culture in 2D plates quickly reaches its limitations with respect to an in-vivo like expression and function of individual cell types. This study investigates whether the VEGF/AMPK pathway regulates autophagy in endothelial cells and whether this is linked to its pro-angiogenic role. Liposomes represent suitable tools for the diagnosis and treatment of a variety of diseases, including cancers. Subgroup. The nut seeds of Garcinia kola are traditionally used in African medicine and known for their anti-microbial, anti-oxidative and anti-inflammatory activities. Analyses of gene and protein expression by qRT-PCR and Western Blot reveal down-regulation of members of the SREBP-pathway. The influenza virus (IV) can cause severe epidemics, which results in significant morbidity and mortality. Although the viral infection originates in the respiratory system, it is unclear how the virus can overcome the alveolar barrier, which is observed in severe COVID-19 disease courses. Email: falk.eilenberger@uni-jena.de Phone: +49 3641 9 47990 The Junior Research Group "Optics in 2D-Materials" is dedicated to the understanding of light-matter-interaction in atomically thin layers of nanomaterials, to the tailoring of their optical response, and to … non-parenchymal cells closely reflecting tissue archit... Background T lymphocytes get in contact with affected organs (e.g., lung) or cells (e.g., erythrocytes), as well as with smoke components and bioactive molecules, whose production is triggered by tobacco smoke. Particle Formulation Group (PFG) Jena Center for Soft Matter (JCSM) Friedrich-Schiller-Universität Jena Philosophenweg 7 07743 Jena Telefon: +49 3641 9 48 599 Wallert, M., et al. You just pay a small fee for courses in the language centre or sports centre, which are not on the curriculum. The emerging organ-on-chip technology has paved the way to model essential liver functions as well as certain aspects of liver disease in vitro in liver-on-chip models. Genes and proteins of the SREBP pathway are down-regulated in monocytes of FH patients. Infections with SARS-CoV-2 lead to mild to severe coronavirus disease-19 (COVID-19) with systemic symptoms. mRNA expression of A: CD36 and B: LRP1 is elevated in monocytes of patients with homozygous or heterozygous FH compared to controls. Friedrich Schiller University, Institute of Microbiology. The Abbe School of Photonics (ASP) provides education programs with multidisciplinary coverage of optical and photonics technology field topics, ranging from current scientific aspects to engineering, with product applications possible in major economic sectors such as information and communication technologies, medicine and health, manufacturing and energy consumption. The most promising combination of markers to predict a patient’s outcome was CaMKIINα and RUNX3. We are a changing group of students of the Friedrich-Schiller-University Jena which is traveling all over the world to learn first hand about diplomacy in MUNs or "Model United Nations". We here report the Although the viral infection originates in the respiratory system, it is unclear how the virus can overcome the alveolar barrier, which is observed in severe COVID-19 disease courses. In this study, we analyzed whether influenza A virus (IAV) and/or Staphylococcus aureus (S. aureus) infections affect SP-A express... AMP-activated protein kinase (AMPK) is activated by vascular endothelial growth factor (VEGF) in endothelial cells and it is significantly involved in VEGF-induced angiogenesis. Alexander S. Mosig. Institute Research Despite intensive research the physiological role and molecular mechanisms of action of the lipophilic antioxidant α-tocopherol (α-TOH(4)) are still poorly understood. (4)Institute of Biochemistry II, Jena University Hospital, Germany; Center for Sepsis Control and Care, Jena University Hospital, Germany. uni-jena.de Editorial. For efficient electroporation detachment of adherent cells is necessary. International Organizations and Crisis Management - IOCM in short - is the masters degree in political science of the Chair for International Relations at the Friedrich-Schiller-University Jena. © 2008-2020 ResearchGate GmbH. The study was undertaken to investigate whether the two major monocyte subsets defined by the surface markers CD14(+)CD16(+) and CD14(++)CD16(-) show differences in their responses to hypercholesterolemia. joachim [dot] denzler [at] uni-jena [dot] de: Phone: +49 3641 946420: Consultation hour: Monday, 2-3 pm (during the lecture periods) Postal Address. Bonn, 2001. It has been suggested that taurolidine has no severe side-effects, but after long-term use morphological and functional changes of the liver were reported. The VBM toolboxes are a collection of extensions to the segmentation algorithm of SPM2, SPM5, and SPM8 (Wellcome Department of Cognitive Neurology) to provide voxel-based morphometry (VBM). Image: Jan-Peter Kasper (University of Jena) The Abbe Center of Photonics (ACP) hosts fundamental and applied research in optics and photonics. The Faculties of Friedrich Schiller University Jena The University is organised into ten Faculties: (10)Institute of Biochemistry II and Center for Sepsis Control and Care, University Hospital Jena, Jena, Germany. We show that VEGF lead... With UC2 (You-See-Too) we present an inexpensive 3D-printed microscopy toolbox. Microorganisms 2020, 8, 577 4 of 18 HA-D222-mpJena/5258 was obtained after three plaque-purification steps [27] from the lung of BALB/c mice infected with the A/H1N1/pdm09 influenza virus isolate A/Jena/5258/09 (kindly provided Julia Dinger. In an earlier study, we identified multiple genes as hypermethylated in tumors of patients with poor prognosis. A prototypic substance is the anti-tuberculosis agent INH, which is metabolized into N-acety... Organs-on-a-Chip werden immer häufiger als ethisch vertretbare Alternative zu Tierversuchen genannt und wurden kürzlich vom World Economic Forum zu den “Top 10 Emerging Technologies in 2016“ gezählt. Upper whisker shows the 90th, the lower whisker the 10th percentile. Genome-wide analyses of hypermethylated CpG-islands in resistant cell lines in combination with qRT-PCR analyses were used to identify epigenetically silenced genes. The fluorescent dye, even when coupled to a nanoparticle, mimics... For many problems in system biology or pharmacology, in-vivo-like models of cell-cell interactions or organ functions are highly sought after. However, it remains a challenge to screen for new and effective nanoparticle-based systems in vitro. karolin.heinze@med.uni-jena.de. 3, Room 4.150, 4th floor Phone: +49 (0) 3641 / 9-43160 Fax: +49 (0) 3641 / 9-43163 G.Steinborn@wiwi.uni-jena.de Chair's Office. We propose... Genetic aberrations are required for the progression of HPV-induced cervical precancers. Microfluidically supported biochip design for culture of endothelial cell layers With improved perfusion conditions. The system is based on concepts of modular development, rapid-prototyping and all-time accessibility using widely available off-the-shelf optic and electronic components. Marko Gröger. Moreover, testing of potential drugs is time-consuming and cost-intensive. Electronic address: Alexander.MOSIG@med.uni-jena.de. However, only few studies exist so far regarding their suitability to detect the effects of drugs requiring a preceding activation via the cytochrome P450 (CYP) system. She holds an MA in com… Author information: (1)Center for Sepsis Control and Care, Jena University Hospital, 07747, Jena, Germany. Room: 08N05 Phone: +49-3641-9-46480 Adverse metabolic effects were observed in tissues and single cells. E‐mail: sandy.mosig@mti.uni‐jena.de Search for more papers by this author The liver organoid is composed of vascular and hepatocyte cell layers integrating Molecular Hemostaseology, Friedrich‐Schiller‐University of Jena, Jena, Germany. Press, A. T., et al. OA (2016) 3(2), FSO130 future science grou Editorial Mosig fluidic devices for … Figure 4A–D in this file shows increased expression of CD36 and CD91/Lrp1 on monocytes of FH patients. Important factors influencing the behavior of nanoparticles in vivo cannot be simulated in scree... Cell-based assays and organ-like substrates gather increasing attention due to their potentials in diagnostic and drug development. Societies Systemic vitamin E metabolites have been proposed as signaling molecules, but their physiological role is unknown. Email: isabelle.staude@uni-jena.de Phone: +49 3641-9-47330. Author information: (1)Department of Gynecology, Jena University Hospital-Friedrich Schiller University Jena, 07747 Jena, Germany. Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute. (2)Institute of Forensic Medicine, Jena University Hospital, 07747, Jena, Germany. The actin cytoskeleton is central for cellular homeostasis and is targeted for anti-cancer chemotherapy. Gröger M(1), Dinger J(2), Kiehntopf M(3), Peters FT(2), Rauen U(4), Mosig AS(1). The current in vitro gut models that... Alterations of the microbial composition in the gut and the concomitant dysregulation of the mucosal immune response are associated with the pathogenesis of opportunistic infections, chronic inflammation, and inflammatory bowel disease. B: Whisker box plots of log2 transformed microarray raw data. alexander.mosig@med.uni-jena.de. The intestine is the primary reservoir of Candida albicans that can cause systemic infections in immunocompromised patients. Long-chain metabolites of α-tocopherol occur in human serum and inhibit macrophage foam cell formation in vitro. Functional Particles (FP) Research topic. Taurolidine has been used for peritonitis, oncological and catheter-lock treatment because of its anti-inflammatory properties. We will see you in Jena in the winter semester 2020/21—partly on site, partly online 10 July 2010 10 July 2010 Educational operations of the winter semester 2020/21 at the Friedrich Schiller University will start on 2 November 2020 and will be conducted—at least partially—on site. jena.de/Karriere or write an Email to alexander.mosig@med.uni-jena.de Reference number: DM23/2020 (internal and external) Application deadline: 30. PD Dr. Alexander Mosig Institute for Biochemistry II und Center for Sepsis Control and Care, Universitätsklinikum Jena „Human microphysiological systems as in vitro platform for emulation of gut, liver, and lung in disease modelling and infection research “ Wednesday Feb 20th, 11:15 BFS, Seminar Room 17.1/2 Schubertstr. Mechanical stimulation of the endothelial monolayer induces morphological remodeling in its cytoskeleton... Hemodynamic forces generated by the blood flow are of central importance for the function of endothelial cells (ECs), which form a biologically active cellular monolayer in blood vessels and serve as a selective barrier for molecular permeability. Alexander S. Mosig1,2 1Center for Sepsis Control and Care, Jena University Hospital, Jena, Germany 2Institute of Biochemistry II, Jena University Hospital, Jena, Germany 3fzmbResearchCentreofMedical Technology and Biotechnology, Bad Langensalza, Germany 4Arthrospec GmbH, Erfurt, Germany Research Article Novel approach for the prediction of cell Aim was to identify methylated genes with functional involvement in cisplatin-resistance development of epithelial ovarian cancer (EOC). Identifikation von Hochfettdiät-induzierten inflammatorischen Gennetzwerken assoziiert mit der Suppression anti-inflammatorischer Phospholipide in humanem Fettgewebe in der NUGAT (NutriGenomics Analysis in Twins) Studie, Sensor enhanced microfluidic devices for cell based assays and organs on chip. 07743 Jena Site plan +49 3641 9-43000 +49 3641 9-43002 dekanat@wiwi.uni-jena.de Opening hours: By appointment Students Junior scientists Staff Alumni & Patrons Researchers Share this page. Institute of Biochemistry IIUniversity Hospital JenaNonnenplan 2-407743 JenaPhone: +49 3641 9 390 910Fax:      +49 3641 9 396 402alexander.mosig@med.uni-jena.deHomepage. Please use the string “Application for PD position – SURNAME NAME” in the email subject and use the PDF format for all your files. Preparation, modification and characterization of polymeric nanoparticles for targeted drug delivery applications ); nderim_kryeziu@hotmail.com (N.K. Raasch, M., et al. Quality control of microarry raw data. Raasch M, Rennert K, Jahn T, Peters S, Henkel T, Huber O, Schulz I, et al. It cross-links the activities of its principal and visiting scientists together with around 300 doctoral and more than 150 Master's degree students. Since 2015 Elected member of the Board of Directors of the CSCC. Benefactors supporting the University. Cigarette smoke components have a proven negative influence on human health. Model United Nations conferences simulate the United Nations, international organizations and state institutions, for instance the WHO, the African Union and ministries. We show that FKN triggers different effects in CD16(+) and CD16(-) monocytes, the two major subsets of human monocytes. Last update: June 2, 2020 Due to the Corona pandemic, the courses in the current summer term 2020 and also in the upcoming winter term 2020/2021 will be online courses. 17 6 Center for Electron Microscopy, Jena University Hospital, Ziegelmuehlenweg 1, D-07743 Jena, Germany 18 19 20 * Correspondence: Dr. Stefanie Deinhardt-Emmer 21 Institute of Medical Microbiology, 22 Jena University Hospital, 23 Am Klinikum 1, 24 D-07747 Jena, Germany 25 26 Email: stefanie.deinhardt-emmer@med.uni-jena.de Due to our privacy policy, only current members can send messages to people on ResearchGate. Research Award of the Federal Ministry of Food and Agriculture for the development of organ-on-chip models as replacement method for animal experimentation, Innovation Award of the BioRegions Germany 2016, Animal Welfare Award of the Federal State of Thuringia 2014. Chair's Office. Institut für Biochemie II Universitätsklinikum Jena Nonnenplan 2-4 07743 Jena Telefon: +49 3641 9 390 910 Telefax: +49 3641 9 396 402 alexander.mosig@med.uni-jena.de Address: 1Molecular Hemostaseology, Friedrich-Schiller-University of Jena, Bachstrasse 18, 07743 Jena, Germany, 2Institute of Molecular Cell Hepatic α-TOH catabolism results in intermediate formation of long-chain metabolites (α-LCMs(5)) α-13'-hydroxy- and α-13'-carboxychromanol (α-13'-OH(6) and α-13'-COOH(7)). Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism. Institute of Biochemistry II University Hospital Jena Nonnenplan 2-4 07743 Jena Phone: +49 3641 9 390 910 Fax: +49 3641 9 396 402 alexander.mosig@med.uni-jena.de Lehrstuhl für Digitale Bildverarbeitung Friedrich-Schiller-Universität Jena 07737 Jena, Germany . There are no tuition fees at the Friedrich Schiller University Jena. Gerhard G. Paulus. Memberships. Evaluation of epigenetic mechanisms in a septic liver model. establishment and characterization of a microfluidically supported in vitro organoid model of the Contrary to the assumption that ACE levels are stable, circulating ACE has been shown to be altered in obesity and weight loss. Beyond the virulence of the virus itself, epidemiological data suggest that bacterial co-infections are the major cause of increased mortality. Analysis of gene expression by qRT-PCR for CD36 and LRP1. Chemisch-Geowissenschaftliche Fakultät der Universität Jena, Unter dem Dach der Chemisch-Geowissenschaftlichen Fakultät sind an der Friedrich-Schiller Universität Jena die Fachrichtungen Chemie, Geologie, Mineralogie, Geophysik und Geographie vereint. & Care, Jena University Hospital, Jena, Germany and InstituteofBiochemistryII, Jena University Hospital, Jena, Germany Alexander.MOSIG@med. (11)Institute of Pharmacy, Friedrich Schiller University Jena, Philosophenweg 14, D-7743 Jena, Germany. (2009). Reducing blood clots through nanostructured surfaces Materials scientists help to improve implants through nanostructured material surfaces Learn more The data provides show an increased lipid content in monocytes of FH patients compared to healthy individuals. Here, we established a new microfluidically supported, biochip-based model of the brain endothelial barrier in c... Fractalkine (FKN, CX3CL1) is a regulator of leukocyte recruitment and adhesion, and controls leukocyte migration on endothelial cells (ECs). We here report the establishment of a liver organoid that integrates NPCs in a vascular layer composed of endothelial cells and tissue macrophages and a hepatic layer comprising stellate cells co... We have established an electroporation protocol for transfection of premature adherent human THP-1 macrophages using Lonza Nucleofector technology. The centers of the boxes represent the median of the genes, upper and lower ends of the boxes represent the 75th and 25th quartiles, respectively. Petra joined Jena University Hospital (JUH), Department of Anaesthesiology and Intensive Care Medicine, in November 2016 as senior academic clinical lecturer to connect her research interests and expertise with the infection research at JUH and its integrated research and treatment Centre for Sepsis Control and Care (CSCC).